HealthData.Gov
An analysis of numerous Drosophila microarray experiments reveals that the genome has many large groups of adjacent genes that are expressed similarly but are not functionally related.
Updated Oct 29, 2026
HealthData.Gov
Background Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRFl and eRF3. eRFl recognizes nonsense codons UAA, UAG and UGA, while eRF3 stimulates polypeptide…
Updated Oct 29, 2026
HealthData.Gov
Background Flavopiridol, a flavonoid currently in cancer clinical trials, inhibits cyclin-dependent kinases (CDKs) by competitively blocking their ATP-binding pocket. However, the mechanism of action of flavopiridol as…
Updated Oct 29, 2026
HealthData.Gov
Background Nocturnin was originally identified by differential display as a circadian clock regulated gene with high expression at night in photoreceptors of the African clawed frog, Xenopus laevis. Although encoding a…
Updated Oct 29, 2026
HealthData.Gov
It is not known to what extent glucocorticoid hormones cause their anti-inflammatory actions and their undesirable side effects by the same or different molecular mechanisms. Glucocorticoids combine with a cytoplasmic…
Updated Oct 29, 2026
HealthData.Gov
Meaningful exchange of microarray data is currently difficult because it is rare that published data provide sufficient information depth or are even in the same format from one publication to another. MAGE will help…
Updated Oct 29, 2026
HealthData.Gov
Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for normalization leads to…
Updated Oct 29, 2026
HealthData.Gov
Background Though the dysfunction of central dopaminergic system has been proposed, the etiology or pathogenesis of schizophrenia is still uncertain partly due to limited accessibility to dopamine receptor. The purpose…
Updated Oct 29, 2026
HealthData.Gov
Background Microarray experiments offer a potent solution to the problem of making and comparing large numbers of gene expression measurements either in different cell types or in the same cell type under different…
Updated Oct 29, 2026
HealthData.Gov
Background Chemical methods of transfection that have proven successful with cell lines often do not work with primary cultures of neurons. Recent data, however, suggest that linear polymers of the cation…
Updated Oct 29, 2026
HealthData.Gov
Background Depending on their larval environment, female honeybees develop into either queens or workers. As in other polyphenisms, this developmental switch depends not on genomic differences between queens and workers…
Updated Oct 29, 2026
HealthData.Gov
Fold-change' cutoffs have been widely used in microarray assays to identify genes that are differentially expressed. More accurate measures are required to identify high-confidence sets of genes with biologically…
Updated Oct 29, 2026
HealthData.Gov
Relative mRNA levels from maize organs have been quantified by hybridization to maize arrays fabricated with 5,376 unique expressed sequence tag clones. mRNAs from embryos, three vegetative organs (leaf blades, leaf…
Updated Oct 29, 2026
HealthData.Gov
Background A key assumption in the analysis of microarray data is that the quantified signal intensities are linearly related to the expression levels of the corresponding genes. To test this assumption, we…
Updated Oct 29, 2026
HealthData.Gov
Background We propose a new method for supervised learning from gene expression data. We call it 'tree harvesting'. This technique starts with a hierarchical clustering of genes, then models the outcome variable as a…
Updated Oct 29, 2026
HealthData.Gov
Background Rapid, robust and reversible induction of transgene expression would significantly facilitate cancer gene therapy as well as allow the in vivo functional study of newly discovered genes in tumor formation and…
Updated Oct 29, 2026
HealthData.Gov
Background: Homeotic genes are key developmental regulators that are highly conserved throughout evolution. Their encoded homeoproteins function as transcription factors to control a wide range of developmental…
Updated Oct 29, 2026
HealthData.Gov
A robust semi-parametric normalization technique has been developed, based on the assumption that the large majority of genes will not have their relative expression levels changed from one treatment group to the next,…
Updated Oct 29, 2026
HealthData.Gov
Background Methods for extracting useful information from the datasets produced by microarray experiments are at present of much interest. Here we present new methods for finding gene sets that are well suited for…
Updated Oct 29, 2026
HealthData.Gov
Background Gene expression is affected by population density. Cell density is a potent negative regulator of cell cycle time during exponential growth. Here, we asked whether SV40 large T antigen (Tag) levels, driven by…
Updated Oct 29, 2026