HealthData.Gov
Background Members of a class of bHLH transcription factors, namely the hairy (h), Enhancer of split (E(spl)) and hairy-related with YRPW motif (hey) (h/E(spl)/hey) genes are involved in vertebrate somitogenesis and…
Updated Oct 29, 2026
HealthData.Gov
Background Data from thousands of transcription-profiling experiments in organisms ranging from yeast to humans are now publicly available. How best to analyze these data remains an important challenge. A variety of…
Updated Oct 29, 2026
HealthData.Gov
Background cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol…
Updated Oct 29, 2026
HealthData.Gov
Background Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity, the method has not been thoroughly…
Updated Oct 29, 2026
HealthData.Gov
Background Homeobox genes of the orthodenticle (otd)/Otx family have conserved roles in the embryogenesis of head and brain. Gene replacement experiments show that the Drosophila otd gene and orthologous mammalian Otx…
Updated Oct 29, 2026
HealthData.Gov
Background Drug resistance in breast cancer is a major obstacle to successful chemotherapy. In this study we used cDNA microarray technology to examine gene expression profiles obtained from fine needle aspiration (FNA)…
Updated Oct 29, 2026
HealthData.Gov
Background It has been recognized that replicates of arrays (or spots) may be necessary for reliably detecting differentially expressed genes in microarray experiments. However, the often-asked question of how many…
Updated Oct 29, 2026
HealthData.Gov
Background The power of DNA microarrays derives from their ability to monitor the expression levels of many genes in parallel. One of the limitations of such powerful analytical tools is the inability to detect certain…
Updated Oct 29, 2026
HealthData.Gov
Background The gene expression pattern in tumor cells differs from that in corresponding normal cells. In order to identify differentially expressed genes in colorectal tumors and normal colorectal epithelium, a…
Updated Oct 29, 2026
HealthData.Gov
Background We performed a statistical analysis of a previously published set of gene expression microarray data from six different brain regions in two mouse strains. In the previous analysis, 24 genes showing…
Updated Oct 29, 2026
HealthData.Gov
Heat shock protein gene expression and survival in critical illness
Updated Oct 29, 2026
HealthData.Gov
Background Drugs & used in anticancer chemotherapy have severe effects upon the cellular transcription and replication machinery. From in vitro studies it has become clear that these drugs can affect specific genes, as…
Updated Oct 29, 2026
HealthData.Gov
Background DAZAP1 (DAZ Associated Protein 1) was originally identified by a yeast two-hybrid system through its interaction with a putative male infertility factor, DAZ (Deleted in Azoospermia). In vitro, DAZAP1…
Updated Oct 29, 2026
HealthData.Gov
Background Previously, we reported effects of the cryb mutation on circadian rhythms in period and timeless gene expression within isolated peripheral Drosophila tissues. We relied on luciferase activity driven by the…
Updated Oct 29, 2026
HealthData.Gov
Background Although the genetics of hybrid sterility has been the subject of evolutionary studies for over sixty years, no one has shown the reason(s) why alleles that operate normally within species fail to function in…
Updated Oct 29, 2026
HealthData.Gov
Background Microarray technologies are emerging as a promising tool for genomic studies. The challenge now is how to analyze the resulting large amounts of data. Clustering techniques have been widely applied in…
Updated Oct 29, 2026
HealthData.Gov
Background Receptor protein tyrosine phosphatase rho (RPTPρ, gene symbol PTPRT) is a member of the type IIB RPTP family. These transmembrane molecules have been linked to signal transduction, cell adhesion and neurite…
Updated Oct 29, 2026
HealthData.Gov
Background Proximal chromosome 15q is implicated in neurodevelopmental disorders including Prader-Willi and Angelman syndromes, autistic disorder and developmental abnormalities resulting from chromosomal deletions or…
Updated Oct 29, 2026
HealthData.Gov
Background Tetracycline-regulated systems have been used to control the expression of heterologous genes in such diverse organisms as yeast, plants, flies and mice. Adaptation of this prokaryotic regulatory system…
Updated Oct 29, 2026
HealthData.Gov
Early changes in transcriptional profiles of circulating mononuclear cells were compared with those occurring within the microenvironment of melanoma metastases following systemic IL-2 administration. The results…
Updated Oct 29, 2026