Datasets

4 datasets

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HealthData.Gov

Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses

Background Post-transcriptional gene silencing (PTGS) by short interfering RNA has opened up new directions in the phenotypic mutation of cellular genes. However, its efficacy on non-nuclear genes and its effect on the…

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Updated Oct 29, 2026

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Reducing hypothalamic AGRP by RNA interference increases metabolic rate and decreases body weight without influencing food intake

Background Several lines of evidence strongly suggest that agouti-related peptide (AGRP) plays a key role in the regulation of metabolic function but ablation of the AGRP gene has no apparent effect on metabolic…

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Updated Oct 29, 2026

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Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in

RNAi can be achieved by feeding worms Escherichia coli expressing dousble-stranded RNA corresponding to a specific gene. An optimized feeding method is presented that results in phenotypes at least as strong as those…

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Updated Oct 29, 2026

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Doxycycline-induced expression of sense and inverted-repeat constructs modulates

Background A tetracycline-regulated (conditional) system for RNA interference (RNAi) would have many practical applications. Such a strategy was developed using RNAi of the gene for phosphogluconate mutase (Pgm). Pgm is…

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Updated Oct 29, 2026